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Full Figure and legend (14K) TopDiscussionThe aim of this study was to adapt an energy transfer assay to the detection of protein ubiquitination in living cells. Using arrestin as a model, we demonstrated that BRET can specifically detect both basal and dynamically regulated ubiquitination processes. Because it cannot be incorporated into proteins, the concentration of free GFP2 UbiAA available for random collision should be equivalent to that reached when the GFP2 moiety alone is expressed, with both being much higher than that attained with GFP2 Ubi for equivalent GFP2 constructs.

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Ducks and moorhens do their thing in the little creek that runs beside the path, the last of the monarch butterflies feed on a swan plant and once again the magic of the place casts its spell on all of us.The waterfall halfway along is a delightful surprise.Several paths offer exits away from the stream and we take the one that leads to Phyllis St Reserve. This, in turn, takes us into Harbutt Reserve and here we leave the walkway and turn into Harbutt Ave (for those who want to continue following the path of Oakley Creek, it runs through a series of reserves all the way to Mt Roskill).Harbutt Ave leads us on to Woodward Rd, across the railway lines and across New North Rd to the beginning of Richardson Rd.Tracks off the road lead into woodland that in spring is riotous with a species of large forget me nots.Near the archery field is a sign pointing down the slope to Toroa Tce.www.ray-banbaratas.top We climb the stile and walk down the path to Mt Albert Rd, where we turn left and walk for about 100m until we can cross into Alberton Ave.We pass Mt Albert Grammar School and Mt Albert Aquatic Centre to reach the little centre of excellence that has arisen opposite Rocket Park playground.

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